Name: StemMACS™ NeuroD1 mRNA, human
Availability: InStock

Product overview

StemMACS™ NeuroD1 mRNA encodes the neural transcription factor NeuroD1 for transient transfection.

Applications

Neuronal differentiation of pluripotent stem cells^1,2
Conversion of fibroblasts into induced neuronal (iN) cells³

Background information

NeuroD1 is a member of the basic helix-loop-helix (bHLH) family of transcription factors. It forms heterodimers with other bHLH proteins and activates transcription by binding to the E-box motif. NeuroD1 is a regulator of insulin expression. In pluripotent stem cell research, ectopic expression of NeuroD1 has been used to induce neuronal differentiation.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.

StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Product data and images

Figure 1

NeuroD1 expression after mRNA transfection:

Human BJ fibroblasts were transfected with 150 ng StemMACS™ NeuroD1 mRNA and 75 ng StemMACS Nuclear eGFP mRNA. 24 hours post transfection, NeuroD1 expression (red) and colocalization with nuclear eGFP (green) were analyzed by immunofluorescence microscopy.

Transfection control	NeuroD1-transfected cells

Scientific resources

Selected references
1. Pang et al. (2011) Induction of human neuronal cells by defined transcription factors. Nature 476: 220-223

Product options: 1

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