StemMACS™ mCherry mRNA

StemMACS™ mCherry mRNA

StemMACS™ mCherry mRNA encodes the red fluorescent protein mCherry for transient transfection.

Applications

  • Positive control for mRNA transfections
  • Optimization of mRNA transfection protocols
  • Transient labeling of cells by transfection

Background information

mCherry is a red fluorescent protein derived from the
Discosoma sp
. protein DsRed. It offers fast maturation kinetics, pH resistance, and excellent photostability.
1
StemMACS mCherry mRNA has been designed for transient expression of the mCherry protein after transfection. Expression can be easily detected by fluorescence microscopy or flow cytometry. The excitation maximum of mCherry is at 587 nm, its emission maximum at 610 nm.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
  • Selected references

    1. Shaner, N. C. et al. (2004)
      Improved monomeric red, orange and yellow fluorescent proteins derived from
      Discosoma sp.
      red fluorescent protein.
      Nat. Biotechnol. 22(12): 1567-1572
Product options: 1
20 µg
USD 280.00

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