Mouse GM-CSF

Mouse GM-CSF

GM-CSF stands for granulocyte macrophage colony-stimulating factor. Mouse GM-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse GM-CSF can be used for a variety of applications, including:
  • Induction of colony formation of granulocyte/macrophage progenitors in semi-solid medium.
  • In vitro generation of DCs from bone marrow or maturation of CD11c+ splenocytes.
  • Generation of antigen-presenting (DC-like) cells in primary brain cell culture.

Background information

GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of FDC-P1 cells (NIBSC 91/658)
  • premium grade: ≥ 5×
    10
    7
    U/mg
  • research grade: ≥ 1×
    10
    7
    U/mg
  • Selected references

    1. Ait-Oufella et al. (2010) B cell depletion reduces the development of atherosclerosis in mice. J. Exp. Med. 207: 1579-1587
    2. DeLamarter, J.F. et al. (1985)
      Recombinant murine GM-CSF from
      E. coli
      has biological activity and is neutralized by a specific antiserum.
      EMBO J. 4: 2575-2581
    3. Cantini, G. et al. (2012) Immunotherapy against the radial glia marker GLAST effectively triggers specific antitumor effectors without autoimmunity. Oncoimmunology 1(6): 884-893
Product options: 6
E. coli
10 µg
USD 175.00
E. coli
25 µg
USD 270.00
E. coli
10 µg
USD 210.00
E. coli
25 µg
USD 340.00
E. coli
100 µg
USD 700.00
E. coli
1000 µg
USD 2,905.00