Human Oncostatin M IS

Human Oncostatin M IS

Human Oncostatin M IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. IS stands for "improved sequence" and is a variant of Human Oncostatin M with a proprietary amino acid substitution.

Applications

Human Oncostatin M IS can be used for a variety of applications including:
  • Study of hematopoiesis, osteogenesis, and neurogenesis.
  • Expansion of human fibroblasts.

Alternative names

OSM

Background information

Oncostatin M, also known as OSM, is a member of the interleukin 6 (IL-6) related cytokine subfamily that includes IL-6, interleukin 11 (IL-11), leukemia inhibitory factor (LIF), and granulocyte colony-stimulating factor (G-CSF). It is a growth and differentiation factor expressed by activated T cells and monocytes that is involved in hematopoiesis, osteogenesis, and neurogenesis. Oncostatin M induces the expression of cytokines like G-CSF and GM-CSF by endothelial cells. It inhibits the growth of a number of tumor cell lines and promotes the growth of human fibroblasts and smooth muscle cells. Human Oncostatin M is active on murine cells.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation assay using TF-1 cells (NIBSC 93/564)
  • premium grade: ≥ 2×
    10
    7
    U/mg
  • research grade: ≥ 8×
    10
    6
    U/mg
  • Selected references

    1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
USD 190.00
E. coli
25 µg
USD 355.00
E. coli
10 µg
USD 235.00
E. coli
25 µg
USD 455.00
E. coli
100 µg
USD 1,345.00
E. coli
1000 µg
USD 5,450.00