Human M-CSF

Human M-CSF

Recombinant human M-CSF can regulate the proliferation, differentiation, and survival of monocytes, macrophages, osteoclasts and their hematopoietic progenitors . The macrophage colony-stimulating factor (M-CSF) is a potent hematopoietic cytokine that is involved in diverse processes, such as regulation of inflammatory responses, bone resorption, atherosclerosis or brain and placental development. M-CSF recombinant protein can be especially used in differentiation studies, cell culture and functional assays.

Applications

Human M-CSF can be used for a variety of applications, including:
  • Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
  • Differentiation of macrophages from peripheral blood monocytes.
  • Differentiation of osteoclasts from CD14+ monocytes.

Background information

Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of NFS-60 cells (NIBSC 89/512)
  • premium grade: ≥ 2×
    10
    7
    IU/mg
    (typical activity: ≥ 7×
    10
    7
    IU/mg
    )
  • research grade: ≥ 1×
    10
    7
    IU/mg
  • Selected references

    1. Michelet, X. et al. (2015) MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b. J. Immunol. 194(5): 2079-2088
    2. Vogel, S. Z. et al. (2015)
      TCAIM decreases T cell priming capacity of dendritic cells by inhibiting TLR-induced Ca
      2+
      influx and IL-2 production.
      J. Immunol. 194(7): 3136-3146
    3. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
    4. Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
    5. McEwan, W. A. et al. (2013) Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat. Immunol. 14(4): 327-336
    6. Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071-20076
    7. Mire-Sluis, A.R. et al. (1995) The international standard for macrophage colony stimulating factor (M-CSF). Evaluation in an international collaborative study. J. Immunol. Methods 179: 141-151
    8. Wang, C. et al. (2010)
      Innate immune response to
      Mycobacterium tuberculosis
      Beijing and other genotypes.
      PLoS One 5(10): e13594
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
USD 195.00
E. coli
25 µg
USD 390.00
E. coli
10 µg
USD 245.00
E. coli
25 µg
USD 475.00
E. coli
100 µg
USD 865.00
E. coli
1000 µg
USD 3,650.00

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