Human IL-33

Human IL-33

IL-33 stands for interleukin 33. Human IL-33 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-33 can be used for a variety of applications, including:
  • Tʜ2 cell activation and maintenance.
  • Investigation of intracellular signaling of IL-1 family and apoptotic/necrotic pathways.
  • In vitro inhibition of osteoclast differentiation.

Background information

Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 family. Similarly to other IL-1 family members, IL-33 exists in both an un-cleaved (full-length) and a cleaved form, although the proteins responsible of IL-33 maturation are still under investigation. IL-33 is expressed by several cell types including epithelial cells, endothelial cells, and fibroblasts, and binds a specific receptor, also known as IL1RL1, which is expressed on Tʜ2 cells and mast cells. Signaling via IL1RL1 involves receptor accessory protein (IL1RAP) and leads to the expression of type 2 cytokines, such as IL-4, IL-5, IL-13. Via enhancing the activation of Tʜ2 cells, mast cells, and eosinophils, IL-33 sustains the humoral response and contributes to adverse Tʜ2 reactions, such as dermatitis, asthma, and allergies. Besides its primary involvement in the immune system modulation, IL-33 is also expressed in blood epithelium and smooth muscle and plays a role in bone protection. It is unclear whether also the immature form of IL-33 could be biologically active after release, such as during necrosis. Moreover, it was suggested that the full length protein could co-localize in the nucleus and act as transcriptional factor. Recombinant Human IL-33 corresponds to the mature form of human IL-33.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of D10.G4.1 cells
  • premium grade: ≥ 8×
    10
    5
    U/mg
  • research grade: ≥ 4×
    10
    5
    U/mg
Product options: 6
E. coli
10 µg
USD 200.00
E. coli
25 µg
USD 370.00
E. coli
10 µg
USD 245.00
E. coli
25 µg
USD 465.00
E. coli
100 µg
USD 1,110.00
E. coli
1000 µg
USD 4,335.00