Human IL-3

Human IL-3

Recombinant human IL-3 (interleukin 3) can promote proliferation, survival, and differentiation of hematopoietic stem cells and committed progenitor cells of the myeloid or megakaryocyte lineage. This includes cells of the basophilic, megakaryocyte, erythroid, eosinophilic, monocytic, and mast cell lineage. Suitable for use in cell culture, functional assays, and differentiation studies, the recombinant human IL-3 is ideal for research.

Applications

Human IL-3 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro , for example, CD34+ cells from umbilical cord blood2 .
  • In vitro differentiation studies, for example, of B lymphoid progenitors3 .
  • Cultivation of plasmacytoid dendritic cells4 .
  • In vitro expansion of hematopoietic stem cells.
  • Investigation of mast cell or basophil function, for example, basophil interaction with blood vessels5 .
  • Investigation of IL-3–mediated signaling pathways.

Background information

Interleukin 3 (IL-3) is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 91/510)
  • premium grade: ≥ 2×
    10
    6
    IU/mg
    (typical activity: ≥ 2.3×
    10
    6
    IU/mg
    )
  • research grade: ≥ 1×
    10
    6
    IU/mg
  • Selected references

    1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
    2. Rossmanith, T. et al. (2001)
      Interleukin 3 improves the
      ex vivo
      expansion of primitive human cord blood progenitor cells and maintains the engraftment potential of SCID repopulating cells.
      Stem Cells 19: 313-320
    3. Crooks, G. M. et al. (2000)
      IL-3 increases production of B lymphoid progenitors from human CD34
      +
      CD38
      cells.
      J. Immunol. 165: 2382-2389
    4. Tas, S. W. et al. (2007) Noncanonical NF-kappaB signaling in dendritic cells is required for indoleamine 2,3-dioxygenase (IDO) induction and immune regulation. Blood 110: 1540-1549
    5. Lim, L. H. et al. (2006)
      Stimulation of human endothelium with IL-3 induces selective basophil accumulation
      in vitro.
      J. Immunol. 176: 5346-5353
    6. Jing, D. et al. (2010)
      Hematopoietic stem cells in co-culture with mesenchymal stromal cells--modeling the niche compartments
      in vitro
      .
      Haematologica 95(4): 542-550
    7. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
    8. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
    9. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    10. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
    11. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 7
E. coli
10 µg
USD 175.00
E. coli
25 µg
USD 270.00
E. coli
4×25 µg
USD 695.00
E. coli
10 µg
USD 210.00
E. coli
25 µg
USD 340.00
E. coli
100 µg
USD 690.00
E. coli
1000 µg
USD 2,980.00

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