Human IL-13

Human IL-13

IL-13 stands for interleukin 13. Human IL-13 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-13 can be used for a variety of applications, including:
  • Inhibition of monocyte and macrophage activation.
  • B cell activation.
  • NK cell and LAK cell stimulation.

Background information

Interleukin 13 (IL-13) is an immunoregulatory protein, which shares similarity in structure and function with IL-4. IL-13 is mainly secreted by Tʜ2 cells, as well as mast cells and NK cells, and binds a multi-subunit receptor, including the alpha chain of the IL-4 receptor, which downstream activates the signal transducer and activator of transcription 6 (STAT-6). Although IL-13 is involved in the pathogenesis of airway diseases such as asthma, it also plays a protective role for the mucosa, via induction of matrix metalloproteinases (MMPs) and other anti-inflammatory effects. Similarly to IL-4, IL-13 induces humoral responses favoring B cell proliferation and isotype switching to IgE, whereas, on monocytes and macrophages, it counteracts the effects of IL-1β, TNF-α, IL-6, by inhibiting the secretion of inflammatory cytokines and increasing IL-1RA expression. IL-13 acts on hematopoietic differentiation and can synergize with IL-2 in the activation of IFN-γ mediated killer activity of lymphokine activated killer (LAK) cells. The protein sequences of human and mouse IL-13 share 58% identity and are cross species reactive.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 94/622)
  • premium grade: ≥ 4×
    10
    5
    U/mg
  • research grade: ≥ 2×
    10
    5
    U/mg
  • Selected references

    1. Diedrich, C. R. et al. (2013) Monocyte-derived IL-5 reduces TNF production by Mycobacterium tuberculosis-specific CD4 T cells during SIV/M. tuberculosis coinfection. J. Immunol. 190(12): 6320-6328
    2. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
    3. Borriello, F. et al. (2015) IL-3 synergizes with basophil-derived IL-4 and IL-13 to promote the alternative activation of human monocytes. Eur. J. Immunol. 45(7): 2042-2051
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 5
E. coli
10 µg
USD 190.00
E. coli
25 µg
USD 355.00
E. coli
10 µg
USD 235.00
E. coli
25 µg
USD 455.00
E. coli
100 µg
USD 1,070.00