Human EGF

Human EGF

EGF stands for epidermal growth factor. Human EGF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human EGF can be used for a variety of applications, including:
  • Proliferation and differentiation of a wide variety of cell types deriving from ectoderm and mesoderm.

Background information

Epidermal growth factor (EGF) is the prototype of the large family of EGF-like proteins with a common structural motif comprising three intramolecular disulfide bonds. EGF is produced by various cell types like mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney. Production of EGF is induced by testosterone and inhibited by estrogens. EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of 3T3 cells
  • premium grade: ≥ 0.8×
    10
    6
    IU/mg
    (typical activity: ≥ 2×
    10
    6
    IU/mg
    )
  • research grade: ≥ 0.5×
    10
    6
    IU/mg
  • Selected references

    1. Diaz-Guerra E. et al. (2012) Intrinsic cues and hormones control mouse mammary epithelial tree size. FASEB J. 26(9): 3844-3853
    2. Robinson, C. J. and Gaines-Das, R. (1996)
      The international standard for epidermal growth factor (EGF): comparison of candidate preparations by
      in vitro
      bioassays and immunoassays. National Institute for Biological Standards and Control.
      Growth Factors 13: 163-170
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 4
E. coli
100 µg
USD 95.00
E. coli
100 µg
USD 90.00
E. coli
500 µg
USD 245.00
E. coli
1000 µg
USD 410.00

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