Human CD40-Ligand

Human CD40-Ligand

Human CD40-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human CD40-Ligand can be used for a variety of applications, including:
  • Stimulation of B cell activation and proliferation.
  • Stimulation of dendritic cell maturation.
  • Induction of cytokine production in peripheral blood monocytes and T cells.

Alternative names

TRAP, CD154, TNFSF5

Background information

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4
+
T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis.

Quality description

Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of CD19
    +
    B cells
  • premium grade: ≥ 4×
    10
    3
    U/mg
  • Selected references

    1. Spriggs, M.K. et al. (1992) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion. J. Exp. Med. 176: 1543-1550
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 4
E. coli
10 µg
USD 170.00
E. coli
25 µg
USD 245.00
E. coli
100 µg
USD 685.00
E. coli
500 µg
USD 1,655.00