The CytoCapture S40-15 products are designed for cell-based assays and high resolution life cell imaging of adherent cells with the intention to control morphology, localization and 3D conformation of the cells. A uniform array of square type polymer cavities lays on top of the cover glass bottoms of these specialized cell culture and imaging consumables. Individual cavities have a distance from surrounding wall to wall of 40 µm. The wall thickness is 5 µm. Height of the walls is 15 µm. The walls of the cavities errect straight upright from the bottom (F-shape). Seeded cells sediment spontaneously inside the microcavities. The geometry of the cavities allows larger cells (diameter 20-30 µm) to enter. Within these cavities the cells use the side walls as guidance for adherence and cell orientation in three dimensions. The microcavities allow that individual cells can be tracked over days without change of their position. If several cells enter a microcavity (after seeding or proliferation) intensive cell-contacts in threedimensional configuration are established and can be investigated. The technology enables high resolution and intratcellular microscopy of adherent and non adherent cells in life cell experiments and after fixation and staining.