The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes.

Data and images for Dead Cell Removal Kit

Figures

Figure 1

Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide.
A:
B:
Unseparated fraction
Dead cell-depleted fraction
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Figure 1

Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide.
View details

Figure 1

Dead cells were eliminated from cultured cells (Jurkat cells) by labeling of cells with Dead Cell Removal MicroBeads and separation over an LS Column in the magnetic field of a MidiMACS™ Separator. Dead cells were fluorescently stained with propidium iodide.

Figure 2

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Working scheme for the Dead Cell Removal Kit.

Figure 2

Working scheme for the Dead Cell Removal Kit.

Specifications for Dead Cell Removal Kit

Overview

The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes.

Detailed product information

Applications

The Dead Cell Removal Kit has been successfully used for the following applications:
  • Removal of dead cells prior to MACS® Separation of cells
  • Reduction of flow sorting time by elimination of dead cells
  • Elimination of dead cells from cell cultures
  • Improvement of immunocytochemical analysis of frozen cells
  • Preparation of viable, single-cell suspensions from tissue
  • Removal of dead cells from spermatozoa samples
  • Removal of dead cells prior to or following transfection of cells

Columns

MS, LS, XS, or autoMACS
®
Columns.

Resources for Dead Cell Removal Kit

Documents and Protocols

Reviews for Dead Cell Removal Kit

Dead Cell Separation from Single Cell Preparation

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Dead Cell Removal Kit (130-090-101)

We prepare single cells from various tissues for our Genomic studies. We encountered lot of damaged and dead cells in the end from the preparation. This kit works works on MACS principle, fairly simple and easy to use. We use this kit to remove dead cells from the cells suspension as part of our procedure.

Dead Cell Removal Kit

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Dead Cell Removal Kit (130-090-101)

Our lab studies ways to enhance immune responses against HIV infection. As such we perform a lot of in vitro killing assays with different effector cells against HIV infected PBMC. One way to measure the outcome of these assays is to look at the number of dead cells after incubating the effectors and targets together in culture. It is very important to start with healthy cells in the beginning of the assay to ensure trusted results in the end. We use the dead cell removal kit for these types of experiments.

Dead Cell Removal Kit

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Dead Cell Removal Kit (130-090-101)

Our lab studies HIV immunology and the enrichment of live cells for downstream analysis is important for many of our protocols. This kit provides a reliable way to remove dead cells while maintaining a highly enriched population of live cells for downstream applications.

References for Dead Cell Removal Kit

Publications

  1. Kinoshita, N. et al. (2002) Autocrine IL-15 mediates intestinal epithelial cell death via the activation of neighboring intraepithelial NK cells. J. Immunol. 169: 6187-6192
  2. Glander, H.J. et al. (2002) Deterioration of spermatozoal plasma membrane is associated with an increase of sperm lyso-phosphatidylcholines. Andrologia 34: 360-366

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