MACS GMP Recombinant Human M-CSF

MACS GMP Recombinant Human M-CSF

M-CSF stands for macrophage colony-stimulating factor. Human M-CSF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
MACS
®
GMP Recombinant Human M-CSF is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Background information

Macrophage colony-stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages, and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.
2–5

Applications

Human M-CSF can be used for a variety of applications, including:
  • Survival studies and apoptosis assays, for example, using peripheral blood monocytes.
  • Differentiation of macrophages from peripheral blood monocytes.
  • Differentiation of osteoclasts from CD14+ monocytes.

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Biological activity

  • The specific activity is determined by proliferation assay using mouse M-NFS-60 cells according to Mire-Sluis, A. R.
    et al.1
    The proliferation assay was calibrated with the international standard for human M-CSF (NIBSC code 89/512) provided by the WHO/ National Institute for Biological Standards and Control.
  • Specific activity: ≥ 4×
    10
    7
    IU/mg
  • Selected references

    1. Mire-Sluis, A.R. et al. (1995) The international standard for macrophage colony stimulating factor (M-CSF). Evaluation in an international collaborative study. J. Immunol. Methods 179: 141-151
    2. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
    3. Meissner, F. et al. (2010) Inflammasome activation in NADPH oxidase defective mononuclear phagocytes from patients with chronic granulomatous disease. Blood 116(9): 1570-1573
    4. Stanley, E.R. et al. (1997) Biology and action of colony-stimulating factor-1. Mol. Reprod. Dev. 46(1): 4-10
    5. Fixe, P. et al. (1997) Macrophage colony-stimulating-factor (M-CSF or CSF-1) and its receptor: structure-function relationships. Eur. Cytokine Netw. 8(2): 125-136
  • Brochures and posters

  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 3
E. coli
10 µg
USD 490.00
E. coli
50 µg
USD 1,350.00
E. coli
250 µg
USD 3,100.00