MACS GMP Recombinant Human IL-12

MACS GMP Recombinant Human IL-12

IL-12 stands for interleukin 12. Human IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
MACS
®
GMP Recombinant Human IL-12 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells.
IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation, and enhances the cytotoxic activity within these cell populations.
Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the TH 1 rather than the TH 2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity.
It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro
and in
vivo
.
2-4

Applications

Human IL-12 can be used for a variety of applications, including:
  • In vitro differentiation of naive CD4+ T cells towards TH 1 cells.
  • In vitro proliferation and stimulation of cytotoxic activity of NK cells and T cells

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Biological activity

  • The specific activity is determined by induction of IFN-γ secretion by PHA-activated T cells.
    1
    The proliferation assay was calibrated with the reference reagent for human IL-12 (NIBSC code 95/544) provided by the WHO/National Institute for Biological Standards and Control.
  • Specific activity: ≥ 3×
    10
    6
    U/mg
  • Selected references

    1. Wulff, H. et al. (2007) Cloning and characterization of an adenoviral vector for highly efficient and doxycycline-suppressible expression of bioactive human single-chain interleukin 12 in colon cancer. BMC Biotechnol. 7: 35
    2. Lieschke, G.J. et al. (1997)
      Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
      in vivo.
      Nat. Biotechnol. 15: 35-40
    3. Miller, J.M. et al. (2010)
      Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
      in vitro
      and
      in vivo.
      Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
    4. Juelke, K. et al. (2010)
      CD62L expression identifies a unique subset of polyfunctional CD56
      dim
      NK cells.
      Blood 116(8): 1299-1307
  • Brochures and posters

  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 3
5 µg
USD 350.00
25 µg
USD 925.00
100 µg
USD 3,100.00