CD3 antibodies

CD3 antibodies

Clone: REA613
BW264/56 recognizes the human CD3 antigen which is present on mature human T cells, thymocytes, and a subset of NK cells. CD3 is associated with the T cell receptor (TCR) and is responsible for its signal transduction. The CD3 antigen is a complex of five invariable chains: γ, δ, ε, ζ, and η. The epitope recognized by the antibody is located on the ε-chain of the CD3 complex.

Alternative names

T3, CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE

Technical specifications

  • Antigen: CD3
  • Clone: REA613
  • Isotype: recombinant human IgG1
  • Alternative names of antigen: T3, CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE
  • Molecular mass of antigen [kDa]: 21
  • Distribution of antigen: NK cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C (36–46 °F). Do not freeze.
  • Available conjugates: PE, FITC
  • Selected references

    1. Gold, D. P. et al. (1986) Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex. Nature 321(6068): 431-434
    2. Birnbaum, M. E. et al. (2014) Molecular architecture of the αβ T cell receptor-CD3 complex. Proc. Natl. Acad. Sci. U.S.A. 111(49): 17576-17581
    3. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
    4. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    5. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    6. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    7. D'Acquisto, F. et al. (2011)
      CD3
      +
      CD4
      -
      CD8
      -
      (double negative) T cells: saviours or villains of the immune response?
      Biochem. Pharmacol. 82(4): 333-340
    8. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    9. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 2
170-081-054

CD3-FITC

1 mL
USD 460.00
170-081-055

CD3-PE

1 mL
USD 550.00