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Discover our different workflows protocols for untouched isolation of tumor cells from different starting material.
Gentle tumor dissociation is a prerequisite for efficient downstream cell separation, analysis, or culture. gentleMACS™ Technology ensure rapid and standardized dissociation of tissue samples into single-cell suspensions.
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Depletion of non-tumor cells from dissociated tissue is essential for receiving pure tumor cells of interest. Based on comprehensive screenings on primary tumor material, cell lines, and health tissues, we have defined combinations of antibodies recognizing all cells of the tumor microenvironment but not the tumor cells. Using these antibodies coupled to MicroBeads can eliminate >95% of contaminating cells in less than 20 min.
MACS Handbook:
Magnetic cell separation
Related PDFs:
Isolation and analysis of human tumor cells from needle biopsy (application note)
Depletion of mouse cells from dissociated patient-derived xenograft tumors (application note)
Novel methods for the isolation of tumor cells from human, mouse, and xenografted tumors (scientific poster)
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Depletion of unwanted cells from cell populations improves analysis as well as culture of target cells. Fast and easy magnetic separation using MACS MicroBeads allows for accurate downstream analysis avoiding bias caused by contaminating cells.
Scientific publication
MACS Handbook:
Flow cytometry basics
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