Miltenyi Biotec-tested panel 15 (MBTP 15)
This application protocol describes the flow cytometric analysis of neutrophils and eosinophils after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD11b | REA592 | Neutrophils and eosinophils | VioBlue® | 450/50 (violet) |
Viobility™ 405/520 Fixable Dye | - | Viability | - | 525/50 (violet) |
Ly-6G | REA526 | Neutrophils | FITC | 525/50 (blue) |
CD193 (CCR3) | REA122 | Eosinophils | PE | 585/40 (blue) |
CD3e | REA606 | Cell type exclusion | PE-Vio® 615 | 655-730 (blue) |
CD19 | REA747 | Cell type exclusion | PE-Vio 615 | 655-730 (blue) |
CD335 (NKp46) | REA815 | Cell type exclusion | PE-Vio 615 | 655-730 (blue) |
Siglec-F | REA798 | Eosinophils | APC | 655-730 (red) |
CD45 | REA737 | Leukocytes | APC-Vio 770 | 750LP (red) |
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