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Miltenyi Biotec-tested panel 23 (MBTP 23)
This application protocol describes the quantitative analysis of mouse cortical neurons upon isolation and culture using flow cytometry. Mouse brains are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD11b||REA592||Microglia||Vio® Bright FITC||525/50 (blue)|
|Propidium Iodide Solution||-||Viability||-||655-730 (blue)|
* Alternative REAfinity™ Antibody available: ACSA-2 Antibody, anti-mouse, APC, REAfinity
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