Miltenyi Biotec-tested panel 28 (MBTP 28)
This application protocol describes the flow cytometric analysis of virus-specific T cells after enrichment of IFN-γ+ cells from human blood. IFN-γ+ cells were enriched using the CliniMACS® Cytokine Capture System (IFN-gamma) to obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
7-AAD Staining Solution | - | Viability | - | 655–730 (blue) |
CD45 | REA747 | Leukocytes | VioBlue® | 450/50 (violet) |
CD4 | REA623 | T helper cells | VioGreen™ | 525/50 (violet) |
CD3 | REA613 | T cells | FITC | 525/50 (blue) |
CD8 | REA734 | Cytotoxic T cells | APC-Vio® 770 | 750 LP (red) |
IFN-γ | 45-15 | Activation marker | PE | 585/40 (blue) |
CD45RO | REA611 | Memory T cells | PE-Vio 770 | 750 LP (blue) |
CD62L | 145/15 | Naive and memory T cells | APC | 655–730 (red) |
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