Immunophenotyping of human CD4+ and CD8+ T cell activation and differentiation using flow cytometry (panel B)

MBTP 58  = Miltenyi Biotec-tested panel 58

This application protocol describes the analysis of human CD4+ and CD8+  T cell activation and differentiation markers after T cell stimulation. To demonstrate this state-of-the-art panel for T cell activation as well as T cell differentiation of both CD4 T cells and CD8 T cells, PBMCs were stimulated for 48 hours using Miltenyi Biotec’s T Cell TransAct™, human. The state of activation and differentiation was defined by CD45RA, CD38, CD197 (CCR7), and HLA-DR expression.

Protocol

Antibody panel for T cell activation and differentiation

SpecificityClonePurposeFluorochromeDetection filter (nm) (laser)
7-AAD*-Viability-655−730 (blue)
CD45RAREA562T cell activation and differentiationFITC525/50 (blue)
CD4*REA623Helper T cellsVioGreen™525/50 (violet)
CD8*REA734Cytotoxic T cellsAPC-Vio® 770750 LP (red)
HLA-DRREA805T cell activation and differentiationAPC655−730 (red)
CD3REA613T cellsVioBlue®450/50 (violet)
CD38REA572T cell activation and differentiationPE585/40 (blue)
CD197 (CCR7)REA108T cell activation and differentiationPE-Vio 770750 LP (blue) 

* Alternative CE-IVD/ASR reagents available. 

Gating strategy

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Materials

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