Miltenyi Biotec-tested panel 43 (MBTP 43)
This application protocol describes the analysis of the surface expression of chimeric antigen receptors (CARs) on T cells after transduction. To demonstrate the state-of-the-art panel, CAR T cells were generated within 12 days using the CliniMACS Prodigy® T Cell Transduction process. The data was analyzed using Miltenyi Biotec’s automated data acquisition and analysis Express Mode program “CAR T Cell Express Mode Package”.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD3*||REA613||T cells||FITC||525/50 (blue)|
|CD4*||REA623||Helper T cells||VioGreen™||525/50 (violet)|
|CD8*||REA734||Cytotoxic T cells||APC-Vio® 770||750 LP (red)|
|CD19 CAR Detection Reagent, Biotin**||-||CAR+ cells||-||-|
|Biotin||REA746||CAR+ cells||PE||585/40 (blue)|
* Alternative CE-IVD/ASR reagents available.
** Alternative CAR Detection Reagents can be used. Choose a CAR Detection Reagent detectable in the PE channel, e.g., CD19 CAR Detection Reagent, human, Biotin (# 130-129-550), CD22 CAR Detection Reagent, human, Biotin (# 130-126-727), BCMA CAR Detection Reagent, human, Biotin (# 130-126-090), CD33 CAR Detection Reagent, human, Biotin (# 130-127-642), CD19 CAR FMC63 Idiotype Antibody, PE, REAfinity™ (# 130-127-342), or CD19 CAR FMC63 Idiotype Antibody, Biotin, REAfinity (# 130-127-345 or # 130-127-349).
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