Miltenyi Biotec-tested panel 34 (MBTP 34)
This application protocol describes the characterization of CAR T cell persistence and differentiation using flow cytometry. For this, CAR T cells were spiked into whole blood. The data was analyzed using the automated data acquisition and analysis tool Express Mode “CAR_T_Cell_Persistence_h_01”.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|7-AAD Staining Solution||-||Viability||-||655–730 (blue)|
|CD3||REA613||T cells||FITC||525/50 (blue)|
|CD4||REA623||T helper cells||VioGreen™||525/50 (violet)|
|CD8||REA734||Cytotoxic T cells||APC-Vio® 770||750 LP (red)|
|CD45RO||REA611||T cell differentiation||APC||655-730 (red)|
|CD62L||145/15||T cell differentiation||PE-Vio 770||750 LP (blue)|
|CD45RA||REA562||T cell differentiation||VioBlue®||450/50 (violet)|
|Biotin||REA746||CAR T cells||PE||585/40 (blue)|
|CD19 CAR Detection Reagent||-||CAR T cells||-||-|
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