Application protocol

Multicolor flow cytometric analysis of macrophages from mouse spleen

This application protocol describes the flow cytometric analysis of macrophages after spleen dissociation from healthy C57BL/6 mice. Viable single cells from mouse spleens are easily obtained using the gentleMACS™ Technology. For downstream flow cytometric analysis of macrophages, we have designed a validated multicolor flow cytometry panel, using our REAfinity™ Recombinant Antibodies and Viobility™ Fixable Dyes. In addition, we provide an optimized gating strategy for the analysis of macrophages from mouse spleen.

Materials

The following is a listing of reagents, instruments, and consumables needed for each step of this protocol. These products are for research use only. 
Matching products:

Protocol

View timeline

This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.