Application protocol

Isolation and cultivation of oligodendrocytes from adult mouse or rat brain 

 In this application protocol, we describe a protocol to generate highly purified and viable oligodendrocytes from adult mouse or rat brain tissue. Brain tissue from mice or rats older than P7 is dissociated into single-cell suspensions using the Adult Brain Dissociation Kit. The extracellular matrix is enzymatically digested using the kit components, while the gentleMACS™ Dissociator with Heaters is used for the mechanical dissociation steps during the on-instrument enzyme incubation. After the dissociation, the myelin and cell
debris are removed using the Debris Removal Solution and is followed by an subsequent removal of erythrocytes using the Red Blood Cell Removal Solution. The Anti-O4 MicroBeads are used to isolate oligodendrocytes from the single-cell suspension.

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Protocol

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This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.

Materials

The following is a listing of reagents, instruments, and consumables needed for each step of this protocol.  These products are for research use only.
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