Enrichment of human carcinoma cells for sensitive molecular analysis 

FFPE Tissue Dissociation Kit from Miltenyi Biotec

BERGISCH GLADBACH, October 30, 2019 – The FFPE Tissue Dissociation Kit by Miltenyi Biotec enables the effective dissociation of formalin-fixed paraffin-embedded (FFPE) carcinoma samples for the specific enrichment and subsequent molecular analysis of carcinoma tumor cells. This enhances the sensitivity in molecular experiments, such as mutation analysis by next-generation sequencing (NGS).

Miltenyi Biotec’s FFPE Tissue Dissociation Kit in combination with the gentleMACS™ Octo Dissociator with Heaters provides a standardized protocol for the dissociation of FFPE tissues. In particular, it has been validated for the dissociation of different carcinoma entities for the enrichment of carcinoma tumor cells prior molecular analysis. After dissociation, epitopes of the epithelial cell marker cytokeratin are well preserved. This enables the specific isolation of cytokeratin-positive carcinoma cells from bulk tumor, and ultimately enhances the sensitivity of molecular analysis of carcinomas.


Benefits of the FFPE Dissociation Kit

  • Effective enrichment of tumor cells from dissociated carcinoma samples based on cytokeratin expression
  • Enhanced sensitivity of DNA analysis from carcinomas with very low tumor cell content
  • Standardized workflow for reproducible results

FFPE samples are prepared for molecular analysis using a workflow of only four steps: i) deparaffinization and rehydration of FFPE slices; ii) heat-induced antigen retrieval; iii) mechanical and enzymatic dissociation of tumor samples into single-cell suspensions by the gentleMACS™ Octo Dissociator and the FFPE Tissue Dissociation Kit, and iv) magnetic enrichment of tumor cells based on cytokeratin expression using the autoMACS® Pro Separator and the Anti-Cytokeratin MicroBeads, human. Both tissue dissociation and magnetic cell enrichment are performed in fully automated processes.

The resulting highly enriched tumor cell fractions are easily amenable to sensitive molecular analysis. The FFPE Dissociation Kit was validated for multiple carcinoma types, including lung, colon, prostate, breast, pancreas, and lymph nodes (metastases).

For further information on the FFPE Tissue Dissociation Kit click here.

Meet us at one of the following events

November 7-9, 2019           AMP 2019 Annual Meeting & Expo, Baltimore, MD, US
December 7-11, 2019         ASCB 2019 Annual Meeting, Washington, DC, US

 

About Miltenyi Biotec
Miltenyi Biotec provides products that advance biomedical research and cellular therapy. Our innovative tools support research from basic research to translational research to clinical application. Our 30 years of expertise includes immunology, stem cell biology, neuroscience, and cancer. Miltenyi Biotec has 2,500 employees in 28 countries.

 

Miltenyi Biotec media contact 
Dr. Anja Kohmann 
Miltenyi Biotec GmbH 
press@miltenyibiotec.de