What is the best way to transition my pluripotent stem cells to StemMACS iPS-Brew XF?

Simply seeding your cells in StemMACS iPS-Brew XF, human after splitting will work for many cell lines (supplement with a Rho-associated protein kinase (ROCK) inhibitor such as StemMACS Y27632 as needed to facilitate attachment).

However, the more gentle way is a gradual transition to the new culture media. If your cell line has been in another media environment for a long time or is generally sensitive to change, exposing it to a new media environment and asking for re-attachment at the same time may be too much.

To achieve a gradual transition, expose your culture to StemMACS iPS-Brew XF while colonies are still attached: 1-2 days before the split, replace the media with a 50:50 mixture of StemMACS iPS-Brew XF and the previous media. After the split, use StemMACS iPS-Brew XF only.