What is a REAfinity Antibody? How does it differ from a standard mouse or rat monoclonal antibody?

REAfinity Antibodies are a new generation of engineered flow antibodies. For each specificity, premium antibody screening technology has been applied to identify the clone with the highest binding affinity and specificity. Only the top candidate is selected to become a recombinant engineered antibody (REA). Based on this process, you can feel confident that your REA clone is the superiour antibody choice for your flow experiment.

Additionally, REAfinity Antibodies show virtually no binding to Fcγ receptors (see figure), which is due to a specific mutation on the Fc part of REAfinity Antibodies. Therefore, no FcR blocking step is required using REA clones and you enjoy significant savings on FcR blocking reagents.

Learn more about REAfinity Recombinant Antibodies.

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Staining of cells with a mouse monoclonal antibody or a REAfinity Antibody in the absence or presence of FcR blocking reagent

Human PBMCs were stained with either a PE-conjugated mouse monoclonal antibody (A, B) or a PE-conjugated REAfinity Antibody (C) recognizing CDa. Cells were also stained with CD-FITC and analyzed by flow cytometry on the MACSQuant Analyzer. Staining with the mouse monoclonal antibody was performed either with (A) or without (B) pre-treatment with FcR blocking reagent. No FcR blocking reagent was included prior to staining with the REAfinity Antibody (C).

Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.