What are the recommendations for cell density, medium volume, and culture vessel size when expanding T cells?

To ensure optimal stimulation and cell growth it is important to start the culture with appropriate cell densities and medium volumes. Ideal starting conditions for human T cell expansion are 2.5×10⁶ cells/mL/cm² and for mouse T cells 1×10⁶ cells /mL/cm² for mouse T cells. For more details about cell numbers and medium volumes that are appropriate for starting cultures in commonly used formats of culture plates, dishes, and flasks, please refer to the data sheet.