Is it possible to perform extracellular surface marker staining on an MS Column?

Yes, this is possible using our Inside Stain Kit. Follow the protocol below:

  1. Apply 500 µL of labeled cells onto an MS Column. 
  2. Wash 3 times with 500 µL of PBS.
  3. Apply 100 µL of PBS containing a 1:100 dilution of Viobility 405/452 Fixable Dye and incubate for 15 min at room temperature.
  4. Apply 60 µL of mastermix (containing 10 µL of each antibody plus PBS) and incubate for 15 min at room temperature in the dark.
  5. Apply 500 µL of PBS.
  6. Elute cells with 500 µL of PBS into a  5-mL tube.