Is it possible to label cells with more than one type of MACS MicroBeads at the same time?

Yes, this is possible because all our protocols are standardized. It is important that you use the correct concentration of each type of MACS MicroBeads. Therefore, you will need to reduce the amount of buffer you add to the cell suspension.

For example, if you want to deplete T and B cells from PBMCs using CD3 MicroBeads, human and CD19 MicroBeads, human, follow the instructions in the CD3 MicroBeads data sheet as a base. Changes in the protocol (2.2 Magnetic labeling) compared the original version are marked in bold:

3. Resuspend cell pellet in 80 μL of buffer per 107 total cells.
Since you are going to add 20 µL of CD19 MicroBeads as well, reduce this amount by 20 µL to 60 µL.

4a. Add 20 µL of CD3 MicroBeads per 107 total cells.

4b. Add 20 µL of CD19 MicroBeads as well.

5. Mix well and incubate for 15 min at 4−8 °C.

You can typically combine up to three different types of MicroBeads. Combining more than three types might lead to difficulties with steric hindrances, so in these cases, we recommend that you use an indirect approach comparable to our isolation kits.