I performed a negative selection and the recovery of my target cells is low. What could be the reason for this?

Check the following factors:

  • Did you incubate at higher temperatures than 4°C or longer than indicated in the data sheet?
  • Did you use more antibody or MACS MicroBeads than indicated in the data sheet?
  • Did you choose the right column? Was the column overloaded?
  • Did you have clumps in your cell suspension? Filter your cells before staining with MACS MicroBeads to avoid clumps.