I performed a negative selection and the purity of my target cells is low. What could be the reason for this?

Check the following factors:

  • Did you choose the right column? Was the column overloaded?
  • Did you incubate for a shorter time or at lower temperatures (e.g., on ice) than indicated in the data sheet?
  • Were there a lot of dead cells in your sample? Dead cells bind unspecifically to antibodies and are therefore co-enriched to a certain extent. Check your sample preparation and try to avoid large numbers of dead cells
  • Did you use less antibody or MACS MicroBeads than indicated in the data sheet?
  • Did you have clumps in your cell suspension? Filter your cells before loading them on the column.