How should I prepare TLR7/8 ligands for use in cell culture experiments?

To enable cellular uptake of TLR7/8 ligands and protect them from degradation, ORNs need to be formulated with cationic lipids. All TLR7/8 ligands from Miltenyi Biotec come with the transfection reagent DOTAP-CI allowing for optimal formulation. To achieve reproducible results, it is important to prepare the ligands according to the instruction indicated in the data sheet which ensures appropriate complex formation.