How can I prepare MACS Separation Buffer?

Prepare a solution by diluting MACS BSA Stock Solution 1:20 with autoMACS Rinsing Solution. Keep the buffer cold (2–8°C).

Alternatively, MACS buffer can be prepared from phosphate-buffered saline (PBS), pH 7.2, 0.5% bovine serum albumin (BSA), and 2 mM EDTA. De-gas the buffer before use, as air bubbles can block the columns.