How can I calculate the recovery of target cells after a separation?

The recovery of cells is often determined by comparing the number of cells isolated with the MACS Cell Separation Technology and the number of cells expected from the heterogeneous starting population.

However, for a more precise calculation, you count the cells in the original fraction, the cells in the target fraction, and the cells in the non-target fraction. The original fraction sample should be taken after magnetic labeling and directly before magnetic separation, as staining and centrifugation steps may lead to cell loss.

Cell recovery can be calculated as follows:

Target cell recovery

Recovery [%] = 100 x ([No. of cells in target fraction] x [% target cells in target fraction]) / ([No. of cells in original fraction] x [% of target cells in original fraction])

Overall cell recovery

Recovery [%] = 100 x ([No. of cells in target fraction + No. of cells in non-target fraction + No. of cells in wash fraction]) / [No. of cells in original fraction]