Do MACSiBead Particles affect downstream applications?

Cells stimulated with antibody-loaded Anti-Biotin MACSiBead Particles can be directly used for any downstream processing such as cytokine analysis or immunoprecipitation. Stimulated cells can also be transfected with high efficiency. Anti-Biotin MACSiBead Particles show no autofluorescence and do not need to be removed prior to flow cytometry. However, when removal of Anti-Biotin MACSiBead Particles is desired, e.g., for restimulation with the particles, this is easily achieved by using the MACSiMAG Separator.