Can I use the same antibody clone for both magnetic and fluorescent labeling?

MicroBead labeling covers only about 1/3 of the available epitopes. Therefore, antibodies with the same specificity can be used for both magnetic and fluorescent labeling without influencing the quality of fluorescent staining. However, dimly expressed antigens, such as the CD133 antigen of hematopoietic progenitor cells or CD25 on regulatory T cells, must be stained with a fluorescent antibody clone different from the one conjugated to the MicroBead. If the same antibody cannot be used, refer to the recommendations on the data sheet.