Can I use MS Columns for depletion?

Our MS Columns have been developed for positive selection of human or animal cells in combination with the MiniMACS Separator or OctoMACS Separator. Nevertheless, they can also be used for depletion of cells that strongly express the magnetically labeled surface antigen.

An MS Column is the column of choice if the total number of cells in the sample is below 1×106.

For all types of columns it is important that you do not exceed the recommended cell number, because this will overload the column, resulting in bad performance. The maximum capacity of an MS Column is 1×107 magnetically labeled cells or 2×108 total cells. If the surface antigen you want to use as the depletion marker does not provide a strong magnetic labeling, we recommend that you use an LS Column or even an LD Column instead.