Live webinar

Isolation of carcinoma cells from FFPE samples for high quality sequencing results

Isolation of carcinoma cells from formalin-fixed, paraffin-embedded (FFPE) samples for high quality sequencing results

Extracting total DNA from FFPE carcinoma samples is a standard technique when looking for sequencing data from these samples. However, up to now it was not possible to distinguish the DNA of carcinoma and non-carcinoma cells in order to improve the quality of sequencing data. We have developed tools that allow for the gentle dissociation of FFPE samples in less time than standard procedures, while preserving epitopes for the subsequent isolation of carcinoma and non-carcinoma cells. In this webinar, we introduce a protocol for the dissociation of a 50 µm FFPE slice and subsequent flow sorting of carcinoma and non-carcinoma cells. Moreover, we present and compare sequencing data obtained from a FFPE carcinoma sample that has been processed either with conventional tools or with our newly developed protocol and tools.

This 60-minute webinar leaves time for questions and discussion.

Presenter: Bastian Nicolai, Miltenyi Biotec, Germany

Register for session 1: June 5, 2018Register for session 2: June 6, 2018
Berlin, Germany (GMT+02:00) – 7:00 PM CET
New York, NY, USA (GMT-04:00) – 1:00 PM EDT
San Francisco, CA, USA (GMT-07:00) – 10:00 AM PST
Berlin, Germany (GMT+02:00) – 10:00 AM CET
London, United Kingdom (GMT+01:00) –  9:00 AM GMT
Singapore, Singapore (GMT+08:00) – 4:00 PM

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