MACS Academy

Stem cell flow cytometry analyses – characterization of pluripotent and differentiated iPS cells

This 2-day training course focuses on the basic principles of human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC) research. Encompassing theoretical presentations and practical lab sessions, the course covers critical steps required for ESC/iPSC research including cell culture and characterization. Participants will hear about ESC/iPSC cultivation techniques and different characterization methods. Furthermore, the training provides hands-on experience in flow cytometric analysis of pluripotent and differentiated iPSCs, introducing a complete workflow for the functional characterization of human pluripotent stem cell lines.

Agenda

Course lectures and exercises include:

  • Basics of pluripotent stem cell cultivation and characterization
  • Basics of flow cytometry
  • Pluripotent stem cell differentiation
  • Discussion and trouble-shooting with experienced users from R&D

Practical laboratory work includes:

  • Hands-on introduction to flow cytometry
  • Compensation of spectral overlap and flow data analysis
  • Quantitative analysis of pluripotent and differentiated iPSCs by flow cytometry

Note: All practical parts are performed using human iPSC lines and iPSC-derived cells.

Learning objectives

  • Understand principles of flow cytometry in the context of iPSC research
  • Become acquainted with ESC/iPSC cultivation and characterization methods
  • Get to know new technologies for iPSC research

The training has a strong focus on practical work, therefore hands-on basic cell culture/laboratory experience is required.

Training dates and locations

MACS Academy Bergisch Gladbach/Cologne, Germany

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