MACS Academy

Basic Flow Cytometry Training

This 2-day training course gives a profound introduction to flow cytometry. Theoretical presentations and hands-on sessions in the laboratory convey the basics of instrument setup, including detector adjustment and fluorescence compensation. The training also outlines the design of multicolor cell analysis experiments, sample staining and measurement, as well as the analysis of flow cytometric data.


All practical flow cytometry procedures are performed on the MACSQuant® Flow Cytometer using the MACSQuantify™ Software.

Agenda

Course lectures, exercises, and lab parts include:

  • Basics of flow cytometry
  • Compensation of the spectral overlap of fluorochromes
  • Setting up a multiparameter cell analysis experiment
  • Data analysis with FlowLogic™ Software

Laboratory practical training includes:

  • Setup of scatter and fluorescence detectors
  • Titration of antibodies
  • Use of dead cell discriminators
  • Compensation and data acquisition
  • Analysis of flow cytometry data, including identification of cell populations and the use of flow cytometric controls

Learning objectives

  • Understand principles of flow cytometry and its applications
  • Design, perform, and analyze a multiparameter flow cytometry experiment, including setting up proper controls and adjusting instrument settings

Training dates and locations

MACS Academy Bergisch Gladbach/Cologne, Germany

MACS Academy San Diego, CA, USA

  • Apr 19, 2018 | more information and registration
  • Aug 16, 2018 | more information and registration (No remaining places.)

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