Miltenyi Biotec blog

REA196 – best CD56 antibody clone for flow cytometry

The human CD56 antigen is a glycoprotein of the Ig superfamily, which is also known as neural cell adhesion molecule (NCAM). CD56 is expressed on practically all resting and activated NK cells and on a minor subset of CD3+ T cells in the blood.

Expression of CD56 is also found in brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal-derived tumors, myelomas, and myeloid leukemias also express CD56.

Clone REA196

Clone REA196, recognizing CD56, makes it the best antibody clone for flow cytometry analysis:

  1. Recombinantly generated under standardized in vitro culture conditions. Recombinant antibodies are highly recommended to achieve scientific reproducibility and consistency.
  2. Genetically engineered Fc region abolishes any background binding to FcyR.
  3. Optimized conjugation chemistry leads to enhanced brightness and stain index in flow cytometry analysis.
View details

Comparison data for clone REA196:

The fluorochrome-conjugated CD56 antibody clones were tested in human PBMCs on a MACSQuant® Analyzer 10.

View details

Antibody titration experiment:

Clone REA196 was compared with two different commercially available antibodies (clone HCD56 and B159). The diagram shows the stain indices of human PBMCs stained at various concentrations of the recommended titer. 

Are you convinced?

Go ahead and find the REA196 antibody conjugated to different fluorochromes in our webshop:

Find all CD56 antibodies


Or see how Miltenyi Biotec supports your entire NK cell workflow:

Go to NK cell workflow