Anti-His antibodies

Anti-His antibodies

Dilution: 1:50
The monoclonal Anti-His antibody facilitates the fast and convenient detection of His-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The antibody is available as a conjugate with horseradish peroxidase (HRP), FITC, PE, or biotin for fast and convenient analysis of His-tagged fusion proteins. Conjugation of the Anti-His antibody to HRP simplifies Western blot or ELISA analysis as the incubation with secondary antibodies becomes dispensable. After incubation the Anti-His-HRP antibody can be directly detected using commercially available chemiluminescent reagents.

Applications

The monoclonal Anti-His antibody specifically recognizes proteins that contain a polyhistidine tag at their N- and C-terminus. However, the Anti-His-HRP (C-term.) antibody shows higher sensitivity and specificity for C-terminal His-tagged proteins. Conjugation of the Anti-His antibody to HRP enables protein detection in Western blot and ELISA analyses without a secondary antibody. Anti-His antibodies conjugated to FITC and PE enable direct flow cytometry and fluorescent microscopy analyses, while indirect fluorescent labeling of His fusion protein expressing cells is possible with the biotin-conjugated antibody.