Mitochondria Isolation Kit, human

Mitochondria Isolation Kit, human

The Mitochondria Isolation Kit, human facilitates the isolation of functional and viable mitochondria from human cells or tissue. The isolation protocol is based on the renowned MACS Technology, which enables fast isolation of high purity and high yield mitochondria.

Detailed procedure

After cell lysis, mitochondria are magnetically labeled with Anti-TOM22 MicroBeads, human, which bind to the translocase of the outer mitochondrial membrane 22 protein (TOM22). The sample is loaded onto an LS Column placed in a MidiMACS or QuadroMACS Separator. After washing, only magnetically labeled mitochondria are retained on the column. The column is removed from the separator and functional human mitochondria are eluted from the column.

Downstream applications

After isolation mitochondria can be further subjected to Western blot analysis
1,6
or downstream functional assays, including measurement of O
2
, membrane potential, and ATP
4-5,7
, or respiratory control
4
. Growing evidence also suggests that isolated mitochondria are well suited for mitochondrial RNA expression profiling studies
2,3
.
Video

Easy isolation of mitochondria using MACS Technology

It is quicker and easier than ever before to isolate mitochondria using MACS Technology. This advanced solution provides a high yield of functional and pure mitochondria in less than 2 hours.

Product options: 3

Product information

Size
Order no.
Price

Mitochondria MidiMACS Starting Kit, human

  • For research use only
130-094-872
-

Mitochondria QuadroMACS Starting Kit, human

  • For research use only
130-094-833
-

Mitochondria Isolation Kit, human

  • For research use only
for 25
separations
(1)
130-094-532
-

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