µMACS One-step cDNA Labeling Kits

µMACS One-step cDNA Labeling Kits

The µMACS One-step cDNA Labeling Kit facilitates fast and effective mRNA isolation and cDNA synthesis, labeling, and purification, without prior preparation of total RNA. Pure mRNA is directly isolated from cells, tissue, or blood, by magnetic labeling with µMACS Oligo(dT) MicroBeads. Due to the fast reaction kinetics the small (50 nm) µMACS Oligo(dT) MicroBeads instantly bind to mRNA molecules and allow for efficient and reproducible isolation of high-purity mRNA. mRNA isolation as well as subsequent cDNA synthesis, labeling, and purification take place in the same column thus reducing loss of material and increasing the sensitivity of microarray based gene expression analysis.

Detailed procedure

After cell lysis and clearing of the cell lysate, the mRNA is magnetically labeled with µMACS Oligo(dT) MicroBeads. Due to the small size of the MicroBeads, the hybridization to the poly(A)
+
tail of the mRNA molecules is completed within seconds. The sample is loaded onto a µ Column placed in the magnetic field of the thermoMACS Separator. After washing, the magnetically labeled mRNA is retained on the column. The First-strand cDNA Labeling Mix is applied to the mRNA and the thermoMACS Separator is set to 42 °C, enabling efficient reverse transcription and concurrent labeling. After thorough washing, pure and labeled cDNA is eluted from the column.
Product options: 2

Product information

Size
Order no.
Price

µMACS One-step cDNA Labeling Starting Kit

  • For research use only
130-092-521
-

µMACS One-step cDNA Labeling Kits

  • For research use only
for 20
reactions
130-092-443
-