Neurosphere Dissociation Kits

Neurosphere Dissociation Kits

The Neurosphere Dissociation Kit (P) has been developed for the gentle and efficient generation of single-cell suspensions from cultured neurospheres. In less than an hour, the gentle enzymatic dissociation procedure efficiently yields high numbers of viable cells. Dissociated cells are immediately ready for further 
in vitro
 or
in vivo
 applications, such as cell culture and expansion, flow cytometry, fluorescence microscopy, and molecular applications, or for cell separations using MACS
®
 Technology.

Background information

Neurospheres are non-adherent spherical clusters of cells formed when neural stem cells are cultured under appropriate conditions. Effective dissociation is important for effective passaging and subsequent downstream applications.
The Neurosphere Dissociation Kit (P) is based on papain. The antigens GLAST (ACSA-1) and PSA-NCAM used to identify some neural populations are sensitive to papain. If preservation of these antigen epitopes is required for downstream applications, it is more suitable to use in this case the Neural Tissue Dissociation Kit (T) (# 130-093-231).
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Product information

Size
Order no.
Price

Neurosphere Dissociation Kit (P)

  • For research use only
for 100
preparations
130-095-943
-

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