Epidermis Dissociation Kit, mouse

Epidermis Dissociation Kit, mouse

The Epidermis Dissociation Kit has been developed for the gentle and efficient generation of single-cell suspensions from mouse epidermis, e.g. for subsequent isolation of Langerhans cells using the Epidermal Langerhans Cell MicroBead Kit.

Applications

For efficient tissue dissociation, the epidermal cell layer is first removed from the dermal cell layer after an overnight enzymatic digestion. In a second step, the epidermal layer is enzymatically digested and subsequently dissociated into a single-cell suspension, either manually or in an automated fashion by using the gentleMACS™ Dissociator and gentleMACS Octo Dissociator.
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