Epidermis Dissociation Kit, human

Epidermis Dissociation Kit, human

The Epidermis Dissociation Kit, human enables the gentle and efficient generation of single-cell suspensions from human epidermal tissue. The kit has been particularly developed for the isolation of keratinocytes from diverse human skin biopsies.

Background information

Human epidermal tissue can be dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins, which maintain the structural integrity of tissues.
In a first step, the epidermal cell layer is removed from the dermal cell layer after enzymatic treatment over night at 4 °C. The epidermal tissue is then further digested enzymatically and dissociated into a single-cell suspension by using the gentleMACS™ Dissociators.
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