Brain tissue dissociation

Gentle, quick, and efficient neural
tissue dissociation

  • Specific tissue dissociation kits optimized for your starting material
  • Automated dissociation of adult neural tissue
  • Preserved cell integrity and surface epitopes

How to dissociate brain tissues for downstream applications?

Tissue dissociation into single-cell suspensions with high cell viability and a minimum of cell debris is a prerequisite for reliable cell separation, cell culture, and flow cytometry analysis. A dissociation procedure that combines optimized enzymatic treatment using our Tissue Dissociation Kits with gentle mechanical dissociation provided by our gentleMACS™ Dissociators is crucial for the preparation of viable single-cell suspensions. 

gentleMACS DIssociator
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gentleMACS Dissociators

  • Fully automated tissue dissociation at the push of a button

  • Optional heaters for enzyme incubation directly on the instrument for walk-away tissue dissociation 

  • Adapted rotational speed enables functional recovery of very sensitive cell types


Generation of viable, single-cell suspension with the Neural Tissue Dissociation Kit (P)
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Tissue Dissociation Kits

  • Available for different starting materials and different embryonic stages

  • High-yield of viable, single-cell suspensions



Choose the specific tissue dissociation kit optimized for your starting material
Tissue dissociation kitsStarting material
Isolation of all neural cells
Adult Brain Dissociation Kit, mouse and rat
Animal age > P7
Neural Tissue Dissociation Kit (P)Animal age ≤ P7
Neural Tissue Dissociation Kit (T)Animal age ≤ P7
Isolation of neurons only
Neural Tissue Dissociation Kit – Postnatal Neurons
Animal age ≤ P7
Special applications
Brain Tumor Dissociation Kit (P)Brain tumor tissue
Neurosphere Dissociation Kit (P)Cultured neurospheres
Embryoid Body Dissociation Kit, human and mouse
In vitro generated embryoid bodies
Myelin Removal Beads II, human, mouse, rat
Single-cell suspensions
(P) Papain, (T) Trypsin
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Dissociation of adult neural tissue

When working with neural tissue derived from > P7 animals, large amounts of cell debris and erythrocytes often hurdle downstream applications. The Adult Brain Dissociation Kit, mouse and rat comes with debris and red blood cell (RBC) removal solutions that overcome this problem. 

Generation of viable, single-cell suspension with the Adult brain Dissociation Kit, mouse and rat
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Adult Brain Dissociation Kit, mouse and rat 

  • Pure neural cells from adult brain tissue in just 2 hours

  • Fully automated and standardized processes for less hands-on time

  • Functional cells ready for cell separation and downstream analysis


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Webinar
Adult brain dissociation and neural cell isolation

Join Hui Zhang, PhD, and her webinar on the latest options and integrated solutions for neuroscience research, and the use of MACS Technology for adult brain dissociation and neural cell isolation.

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