Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry, human

Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry, human

The Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry is a ready-to-use kit that allows for more than a 10,000-fold enrichment and the subsequent immunocytochemical detection of disseminated epithelial tumor cells from blood, bone marrow, or lymphoid tissue by the positive selection of cytokeratin 7/8 expressing cells.

Background information

Most malignant cells which have their origin in epithelial tissue express cytokeratin and can be recognized by an anti-cytokeratin antibody
1
. With the Carcinoma Cell Enrichment and Detection Kit, disseminated epithelial tumor cells can rapidly and efficiently be enriched using MACS Cytokeratin MicroBeads, e.g. from a frequency of 1 tumor cell in 10
7
leukocytes to a frequency of 1 tumor cell per 10
3
leukocytes. Thus, large patient samples can be narrowed and screened for disseminated tumor cells on only a few microscopy slides.

Detailed procedure

The carcinoma cells are permeabilized, fixed, and magnetically labeled with Anti-Cytokeratin MicroBeads. Magnetically labeled cells are then stained with a highly specific Anti-Cytokeratin-FITC antibody and Anti-FITC Alkaline Phosphatase and subjected to magnetic separation. After separation, cells are spun down onto a glass slide and stained with an alkaline phosphatase substrate.

Columns

For enrichment and in-column staining: MS Columns.
Product options: 1

Product information

Size
Order no.
Price

Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry, human

  • For research use only
for 1×
10
9
total cells
–3×
10
9
total cells
130-060-301
-

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